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Image Search Results
Journal: Cellular and Molecular Life Sciences
Article Title: Role of hsa_circ_0000280 in regulating vascular smooth muscle cell function and attenuating neointimal hyperplasia via ELAVL1
doi: 10.1007/s00018-022-04602-w
Figure Lengend Snippet: Secondary sequencing uncovered CHD-related PBMC circRNAs, validated by qRT-PCR in human atherosclerotic vessels. A Volcano plot of sequenced PBMC circRNAs. X-axis, fold modification expressed as log2; Y-axis: p value expressed as − log10. Horizontal line represents p = 0.05. Compared with the control, red and green points exhibited significantly upregulated and downregulated circRNAs at p < 0.05 and p < 0.05, respectively. Blue point represents hsa_circ_0000280 (CHD, n = 70; control, n = 30; Student t test). B ELAVL1 RIP-sequencing revealed top seven circRNAs. C Abundance of top seven circRNAs in three types of cardiovascular cells ( n = 3). D Expression of three circRNAs measured in PDGF-BB-induced HASMCs ( n = 3, p < 0.01; Student t tests). E Quantitative RT-PCR analysis of hsa_circ_0000280 in human coronary arteries with and without (matched controls) luminal stenosis. ( n = 5 pairs, p = 0.005; Student t test). F , G Fluorescence in situ hybridization assay (FISH) shows location and expression of hsa_circ_0000280 in control and CHD tissues. White arrows indicate intimal layer. Probes for hsa_circ_0000280 were labeled with Cy3. Scale bar, 100 µm ( n = 5 pairs, p = 0.0038; Student t tests). Data are presented as means ± SD
Article Snippet:
Techniques: Sequencing, Quantitative RT-PCR, Modification, Control, Expressing, Fluorescence, In Situ Hybridization, Labeling